I am working on a project that is analyzing the binding of a protein to a polymeric surface and was informed that it could simply be done in the chemical hood or the bench and not the biological hood. I wanted to know if it is commonly a problem for bacteria, fungi, etc. to attach and proliferate on surfaces (such as the polymeric surface) when not in sterilized conditions. In my project, no cells are involved but the Bradford method will be implemented to test for protein concentration and I’m concerned as to whether the presence of even a small amount of bacteria, fungi, etc. can affect my protein concentration readings since this study is not executed in sterile conditions.
Also, the solution in which the protein is dissolved is PBS (pH 5 and 7).