Viewing 2 reply threads
  • Author
    Posts
    • #18015
      w19910411
      Participant

      Why are all my cells in flow cytometry analysis dead??

    • #116019
      Fornita
      Participant

      Your operation process, the temperature, all these could influence the cell, especially your treatment, which does not gentle enough.

    • #116144
      biomind2017
      Participant

      Recently, I used FACS-buffer (PBS, 2 mM EDTA, 25 mM HEPES) for antibody dilution and washing. Although, clump formation was less but it was present. Besides, I would also like to know that do the presence of HEPES buffer and EDTA interfere with the result while making antibody dilution buffer.

Viewing 2 reply threads
  • You must be logged in to reply to this topic.