why do RNA show smear instead of discrete bands?

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    • #13915
      tracyliu
      Participant

      if the isolated DNA is not treated RNase, then RNA are present, but how come RNA molecules don’t get separated like the digested DNA fragments? aren’t they also in different sizes?

    • #101755
      sorin
      Participant

      They get separated like DNA, but remember how many different RNA molecules are in a cell,its not like chromosomes or fragments of restriction digests having a discrete size. Apart from this if you do not carefully prepare your samples RNAse contamination can occur quite often…

    • #101757
      JackBean
      Participant

      as sorin wrote, there are plenty of RNAs in the cell each with different length (even the polyA can only differ)

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