why does taq add an "A"

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    • March 28, 2009 at 11:43 am #11131
      theendperson
      Participant

      Hiya I wanted to know why taq polymerase add an A to the end of a transcript

      Thanks in advance

      Tom

    • March 30, 2009 at 12:15 am #89917
      plasmodesmata11
      Participant

      is that something to do with a poly adenyl tail?

    • March 30, 2009 at 8:27 am #89922
      theendperson
      Participant

      dont think so, as far as I can tell it only adds 1 A

    • March 30, 2009 at 3:43 pm #89926
      canalon
      Participant

      I don’t know why. But it is very convenient for TA cloning. And it seems to be a unique chareacteristic of the Taq polymerase, others like Pfu do not do that. I have never seen any explanation for the why.

    • March 31, 2009 at 3:34 am #89937
      MrMistery
      Participant

      I really doubt it actually has a role, honestly. I think it’s much more probable that it’s simply a result of drift in evolution… But this is just my opinion

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