Biology Forum › Molecular Biology › 2ME and SDS
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- November 15, 2011 at 8:56 am #15687
biology_06erParticipantHi there,
So I’ve recently been tryed to purify my protein and everytime I ran a SDS gel, I had a band at 120kda and my protein is around 30kda. I tried size exlcuion, ion exchange, but that 120 band was allways there…so then it was found out I didn’t add 2ME to my reducing buffer :S…and once it was added, viola, the 120 band was gone. So my question is, if 2ME’s role is to break the disulfide bonds to make a monomeric protein. So in the case the bonds arent broken, the size is 4x–does this mean the protein is made up of 4 subunits?…i don’t really get the fact the a computer program says the estimated MW is 32kda but then this gel says its 120 if bonds arent broken–does it mean my protein is able to form a tetramer but in nature the protein is actually only a monomer?
Hope I make sense!
Thanks
b_06er - November 15, 2011 at 10:44 am #108113canalonParticipant
As far as I can see your problem, it tells you that the computer is unable to guess that the protein will form a tetramer in nature. Hardly surprising that…. The computer will just the size of the sequence you fed it and calculate a size based on that. It does not magically analyse the protein to infer disulfide bonds and such.
- November 15, 2011 at 11:25 am #108116JackBeanParticipant
As canalon said, the program calculates MW of only one subunit, but obviously, they are connected by disulfide bonds to form tetramer.
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