Biology Forum Cell Biology A new protein molecule..

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    • #3739
      ambercote
      Participant

      What is the formula for the production of a new protein molecule??

    • #41510
      canalon
      Participant

      Create the appropriate DNA sequence, insert in a selectable plasmid with a good promoter (plenty are available on the market) transform an innocent E. coli, select and feed and you have plenty of your new protein.
      This is a rather rough sketch to answer a vague question.

    • #41524
      blazerUAB
      Participant

      Try and be a little a more specific. I think I know what you mean but clarify by telling me what you know

    • #41525
      canalon
      Participant

      To be clearer what you need is a gene that will code for the protein, and a translation system. You can try acellular all the way (mix of synthetic RNA and ribosomes with the right buffers and molecules) or more simply put your bioengineered gene in a host as bacteria. You need to choose a vector adapted to your host (bacteria here, but could be eukaryotic cell). A way to select the cells that have your gene is usefull too 🙂

    • #41587
      ambercote
      Participant

      Alright thanks guys.

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