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    • #10816
      Snoopy
      Participant

      Hello everybody. I was just wondering if there any formula to calculate the necessary amount of DNA for effective transformation. Maybe someone know where to look in to get this information. Thank you

      Andrey

    • #88733
      pcrboy
      Participant

      you mean transfection? or cancer transformation?

    • #88734
      Snoopy
      Participant
      quote pcrboy:

      you mean transfection? or cancer transformation?

      I mean, transformation – foreign DNA to host cell.

    • #88735
      pcrboy
      Participant

      not much at all is necessary. for example, commercially available vectors for transformation efficiency control are transformed at the pg level. however, if u are using ligation products, i used something like 50ng.

    • #88737
      Snoopy
      Participant

      Thanks, I also use 50-100ng, but I was wondering maybe there is some formula to count.

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