Amount of DNA for eficient transformation - Biology Forum

last updated by

Snoopy 15 years, 10 months ago

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- January 28, 2009 at 8:11 am #10816
  Snoopy
  Participant
  Hello everybody. I was just wondering if there any formula to calculate the necessary amount of DNA for effective transformation. Maybe someone know where to look in to get this information. Thank you
  Andrey
- January 29, 2009 at 3:30 am #88733
  pcrboy
  Participant
  you mean transfection? or cancer transformation?
- January 29, 2009 at 7:45 am #88734
  Snoopy
  Participant
  quote pcrboy:
  you mean transfection? or cancer transformation?
  I mean, transformation – foreign DNA to host cell.
- January 29, 2009 at 12:10 pm #88735
  pcrboy
  Participant
  not much at all is necessary. for example, commercially available vectors for transformation efficiency control are transformed at the pg level. however, if u are using ligation products, i used something like 50ng.
- January 29, 2009 at 12:13 pm #88737
  Snoopy
  Participant
  Thanks, I also use 50-100ng, but I was wondering maybe there is some formula to count.
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