Biology Forum › Microbiology › ANNTI FUNGI !
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- September 17, 2011 at 2:14 pm #15386
NmrParticipantHI GUYS…
WHAT IS THE BEST ANTI-FUNGI THAT CAN I USE IN MY BACTERIAL CULTURE ?
COZ, I HAD FUNGI IN MY BACTERIAL BROTH MEDIUM !
THANK YOU
- September 17, 2011 at 2:27 pm #106373JackBeanParticipant
1) don’t shout on us
2) I think that pH should be quite enough. Further you can use some antibiotic. - September 19, 2011 at 7:18 pm #106386canalonParticipant
Or just be careful with your culture technique, ie if you did not put a sample that contained a mixed population of micro-organisms in your medium, what you need is proper aseptic techniques rather than anti-fungal. Just saying.
- October 22, 2011 at 3:26 pm #107122chooziParticipant
YOU CAN USE NYSTATIN OR AMPHOTERICIN-B AS ANTIFUNGAL.
- October 22, 2011 at 6:18 pm #107123JorgeLoboParticipant
Agree with canalon. If you can;t maintain aseptic technique and pure cultures – get out of the micro lab.
I’ll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.
- October 24, 2011 at 12:46 am #107191daniel.kurzParticipantquote JorgeLobo:Agree with canalon. If you can;t maintain aseptic technique and pure cultures – get out of the micro lab.
I’ll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.
I agree both above. Use a flame and some ethanol and be careful. Anti-fungals can change microbioal physiology that is not natural and make your experiments not as valid. That grammar is terrible…
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