[Nmr]

HI GUYS…

WHAT IS THE BEST ANTI-FUNGI THAT CAN I USE IN MY BACTERIAL CULTURE ?

COZ, I HAD FUNGI IN MY BACTERIAL BROTH MEDIUM !

THANK YOU

[JackBean]

1) don’t shout on us
2) I think that pH should be quite enough. Further you can use some antibiotic.

[canalon]

Or just be careful with your culture technique, ie if you did not put a sample that contained a mixed population of micro-organisms in your medium, what you need is proper aseptic techniques rather than anti-fungal. Just saying.

[choozi]

YOU CAN USE NYSTATIN OR AMPHOTERICIN-B AS ANTIFUNGAL.

[JorgeLobo]

Agree with canalon. If you can;t maintain aseptic technique and pure cultures – get out of the micro lab.

I’ll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.

[daniel.kurz]

quote JorgeLobo:

Agree with canalon. If you can;t maintain aseptic technique and pure cultures – get out of the micro lab.

I’ll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.

I agree both above. Use a flame and some ethanol and be careful. Anti-fungals can change microbioal physiology that is not natural and make your experiments not as valid. That grammar is terrible…

[Author]

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