Or just be careful with your culture technique, ie if you did not put a sample that contained a mixed population of micro-organisms in your medium, what you need is proper aseptic techniques rather than anti-fungal. Just saying.
Agree with canalon. If you can;t maintain aseptic technique and pure cultures – get out of the micro lab.
I’ll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.
Agree with canalon. If you can;t maintain aseptic technique and pure cultures – get out of the micro lab.
I’ll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.
I agree both above. Use a flame and some ethanol and be careful. Anti-fungals can change microbioal physiology that is not natural and make your experiments not as valid. That grammar is terrible…