ANNTI FUNGI !

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    • #15386
      Nmr
      Participant

      HI GUYS…

      WHAT IS THE BEST ANTI-FUNGI THAT CAN I USE IN MY BACTERIAL CULTURE ?

      COZ, I HAD FUNGI IN MY BACTERIAL BROTH MEDIUM !

      THANK YOU

    • #106373
      JackBean
      Participant

      1) don’t shout on us
      2) I think that pH should be quite enough. Further you can use some antibiotic.

    • #106386
      canalon
      Participant

      Or just be careful with your culture technique, ie if you did not put a sample that contained a mixed population of micro-organisms in your medium, what you need is proper aseptic techniques rather than anti-fungal. Just saying.

    • #107122
      choozi
      Participant

      YOU CAN USE NYSTATIN OR AMPHOTERICIN-B AS ANTIFUNGAL.

    • #107123
      JorgeLobo
      Participant

      Agree with canalon. If you can;t maintain aseptic technique and pure cultures – get out of the micro lab.

      I’ll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.

    • #107191
      daniel.kurz
      Participant
      quote JorgeLobo:

      Agree with canalon. If you can;t maintain aseptic technique and pure cultures – get out of the micro lab.

      I’ll also offer that cycloheximide is used in media where one has to isolate bacteria from a sample also containing fungi. Neither Nystatin nor Ampohtericin se soluble enough to be used in microiologcal media. pH is not a useful variable to prevent fungal growth.

      I agree both above. Use a flame and some ethanol and be careful. Anti-fungals can change microbioal physiology that is not natural and make your experiments not as valid. That grammar is terrible…

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