Biology Forum Community General Discussion AS Bio Yeast CSWK

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      I have a question regarding the coursework, I hope this query doesn’t make me sound downright thick but here we go. The investigation is to find the lowest temperature that kills all the yeast cells in a suspension. Using anaerobic respiration and methylene blue to observe whether yeast cells are alive or dead. I did some research, and found out that yeast cells die when exposed to ethanol concentrations of 15% or above in a solution. If the equation is as follows:

      sugar—–>alcohol, carbon dioxide and energy

      then how would I measure the percentage of ethanol, to make sure that the yeast does not die?

      Or will the concentration not get that high?

      Any advice and pointers would be extremely welcome.

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