Biology Forum › Molecular Biology › AS-PCR

last updated by sali213 13 years, 10 months ago
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    • December 26, 2010 at 10:31 am #14303
      sali213
      Participant

      Hellow,
      please i would know th protocol of AS-pcr and what is th difference between it and ASO-pcr.
      thanks.

    • December 27, 2010 at 1:41 pm #102935
      Kezzer
      Participant

      As far as I am aware AS-pcr and ASO-pcr are the same technique and use allele specific oligonuleotides (primers) in SNP detection.
      There are plenty of protocols available online and on Springerlink for allele specific PCR.

    • December 27, 2010 at 3:38 pm #102937
      vbbgold
      Participant

      I agree with Kezzer

      Here are the links:

      http://en.wikipedia.org/wiki/Allele-spe … nucleotide
      http://www.springerlink.com/content/l03 … 042&page=1

      Hope it helps!!!

    • December 27, 2010 at 7:45 pm #102938
      sali213
      Participant

      thank’s

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