please i would know th protocol of AS-pcr and what is th difference between it and ASO-pcr.
As far as I am aware AS-pcr and ASO-pcr are the same technique and use allele specific oligonuleotides (primers) in SNP detection.
There are plenty of protocols available online and on Springerlink for allele specific PCR.
I agree with Kezzer
Here are the links:
http://en.wikipedia.org/wiki/Allele-spe … nucleotide
http://www.springerlink.com/content/l03 … 042&page=1
Hope it helps!!!