buffer - Biology Forum

last updated by

mith 16 years, 10 months ago

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- April 27, 2008 at 3:00 am #9536
  jojo90
  Participant
  i’ve encountered a qns that i dunno how to do
  hope u guys can help me out thx.
  the qns:
  a student was told to dilute Tris-HCL buffer from 1M to 10mM before using it for a biological assay. Instead of doing that, he used the 1M solution directly in his assay, citing that a higher concentration of the buffer would providebetter buffering capacity.State if you agree with him and why.
- April 27, 2008 at 4:51 am #83704
  mith
  Participant
  The question is asking two things I think–
  1. Is the student’s explanation correct? i.e. does 1M buffer provide better buffering?
  2. If true, is this a good idea?
- April 27, 2008 at 5:31 am #83706
  jojo90
  Participant
  haha thxz but i still dunno how to do
- April 27, 2008 at 2:41 pm #83712
  mith
  Participant
  You can start by looking up or reading up on how a buffer works.
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