buffer

[jojo90]

i’ve encountered a qns that i dunno how to do
hope u guys can help me out thx.

the qns:
a student was told to dilute Tris-HCL buffer from 1M to 10mM before using it for a biological assay. Instead of doing that, he used the 1M solution directly in his assay, citing that a higher concentration of the buffer would providebetter buffering capacity.State if you agree with him and why.

[mith]

The question is asking two things I think–

1. Is the student’s explanation correct? i.e. does 1M buffer provide better buffering?

2. If true, is this a good idea?

[jojo90]

haha thxz but i still dunno how to do

[mith]

You can start by looking up or reading up on how a buffer works.

[Author]

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