Calculating moles from base pairs ??

last updated by

taruntbiotech 12 years, 7 months ago

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- April 17, 2012 at 3:48 pm #16368
  lulubell
  Participant
  Hi,
  I’m trying to figure out the moles of a piece of DNA- using just the following information …..
  The genomic DNA is 20000ng (mass) and is 15000 base pairs long
  I know to get moles you have to do; Mass/ relative formula mass <— but I don’t know this….
  Is it possible to do; mass/base pairs to get the number of moles?
  In which case it would be 20000/15000 = 1.333 M ??
  I then need to find the mass (in ng) in 1 mole- I know how to do this, but obviously if I get the first part wrong, my answer here will be wrong also
  Any advice?
- April 17, 2012 at 8:22 pm #110670
  JackBean
  Participant
  1500 bp is not molecular weight. For this you need average weight of nucleotide and with the number of nucleotides in molecule you calculate the molecular weight of the whole molecule. hat you use to calculate concentration.
  But the concentration of DNA is usually in mass per volume (e.g. ng/ul).
- May 14, 2012 at 5:31 pm #111068
  taruntbiotech
  Participant
  see this hope it will help u
  Single-stranded: 330 grams per mole per base
  330 (g/mol)/b
  330 g / ( mol • b )
  Double-stranded: 660 grams per mole per base pair
  660 (g/mol)/bp
  660 g / ( mol • bp )
  Example problem:
  Find the number of moles which compose a 1.5kb band whose mass was determined to be 120 ng (by gel comparison to standards).
  (120 n g ) • ( mol • bp ) = 1.2E-4 nmol or 0.12 pmol
  (1500 bp) ( 660 g )
- May 14, 2012 at 5:35 pm #111069
  taruntbiotech
  Participant
  molbiol.edu.ru/eng/scripts/h01_07.html
  also check this
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