The following is a Ponceau S stained blot. It shows the elution from your IP with and without bME addition. The antibody used for the IP is specific to a 30 kDa protein which is a monomeric polypeptide.
Which band is the Antigen recognized by the IP antibodies?
I put B and E
How many separate proteins immuno-precipitated as interacting partners of the Antigen?
5Which of the following terms describe the interacting partner(s)?
Monomeric and heterodimeric
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