Biology Forum › Molecular Biology › Can you reuse qiashredder columns?
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- November 5, 2007 at 7:34 pm #8516
science outParticipantOur lab has recently undergone a serious budget crunch and need to cut back on our expenses by about half. We do a lot of RNA extractions using RNeasy kits, DNase treatment and qiashredder columns and get great results. Does anyone have some suggestions for alternate methods of RNA extractions or ways to reuse the qiaShredder columns?
Thanks for the help!
- November 5, 2007 at 8:48 pm #77500science outParticipant
You CAN regenerate DNA and RNA resin columns…here’s how:
hmmm…they won’t let me post a link ‘cuz I’m new, so here the details, you’ll have to look it up yourself: Biotechniques vol. 42 no. 2 2007, Siddappa NB et al "Regeneration of commercial nucleic acid extraction columns without the risk of carryover contamination"
The authors say there is no DNA/RNA carryover contamination and they’ve used the columns multiple times. Have fun!
Now I just need to know if you can reuse qiaShredder columns as well…[/i]
- November 24, 2007 at 3:10 am #78412lizengpengParticipant
wo ca give me the method of regenerate DNA and RNA QIAGEN column!Thanks very much!
- November 26, 2007 at 5:05 pm #78510h2so4hurtsParticipant
All the qiashredder columns do is tear open your tissue, they shouldn’t bind nucleic acid. I don’t see why you can’t just was them really well, or just pull your sample through a 22guage or smaller syringe.
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