Subcultivation Method: The method used to transfer from one culture vessel to another. For example, trypsin-EDTA is a standard subcultivation method for attached cells, such as fibroblasts.

http://locus.umdnj.edu/nia/search/datainfo.html

The ratio would perhaps be the affinity or amount able to adhere or bind with the culture medium? I really don’t know and this is the best that a few minutes on Google yielded, I would too like this matter to be eloborated. The best I could possibly associate is Serial dilutions, which I don’t believe are being described here but do involve similar methods.

hmm i also have the notion that its the adherence fraction…can somebody please clarify..?

Ved

Ha, wow that was a while ago. I haven’t posted since then and this was the first thread I read. Amusing.

I have found several abstracts that entail of such methods. Take a look:

http://www.ncbi.nlm.nih.gov/entrez/quer … &DB=PubMed

I know what the subcultivation ratio is.
When you grow a flask of cells, and the flask is confluent(full of cells), you want to split and grow more. So for example you want to use one flask of cells and you need to have 5 flasks altogether, you will need to use a subcultivation of 1:5.
Because cells need a high enough ratio to grow(too few cells in a flask will not grow), a ratio will be needed to see what is the best amount to put in a flask. What I mean is if the ratio is 1:3 that means it is best to use 1 flask full of cells and grow them into 3 flask. Then you will have enough cells in each of those three flasks for healthy growth.

From cell culture technician