Cell culture - Biology Forum

last updated by

kpatel 18 years, 8 months ago

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- March 1, 2006 at 10:34 pm #3841
  kpatel
  Participant
  Some cell require incubating overnight at 37oC in 7% CO2. Can anyone tell me why do we need 7% CO2 and not 5% CO2 which is normally used.
- March 3, 2006 at 4:59 pm #42283
  2810712
  Participant
  Curious too! Do u know why 5%? Atmospheric % is much lesser…
  photosynthetic cells better have more co2 untill it acidifies them to disturb their activities…
  what kind of cells those 7%s are?
- March 4, 2006 at 1:59 am #42322
  kpatel
  Participant
  While isolating the schwann cells from the sciatic nerve, I need to treat the nerve with enzymes an incubate them at 7% CO2.
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