Biology Forum › Cell Biology › Cell culture

last updated by kpatel 18 years, 1 month ago
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    • March 1, 2006 at 10:34 pm #3841
      kpatel
      Participant

      Some cell require incubating overnight at 37oC in 7% CO2. Can anyone tell me why do we need 7% CO2 and not 5% CO2 which is normally used.

    • March 3, 2006 at 4:59 pm #42283
      2810712
      Participant

      Curious too! Do u know why 5%? Atmospheric % is much lesser…
      photosynthetic cells better have more co2 untill it acidifies them to disturb their activities…
      what kind of cells those 7%s are?

    • March 4, 2006 at 1:59 am #42322
      kpatel
      Participant

      While isolating the schwann cells from the sciatic nerve, I need to treat the nerve with enzymes an incubate them at 7% CO2.

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