Biology Forum Cell Biology cell culture on PDMS

last updated by mith 14 years ago
3 voices
5 replies
  • Author
    Posts
    • #12798
      Sohye
      Participant

      How can I detach cells from surface of PDMS?
      I did trypsin with EDTA treatment for two minutes but, it didn’t work well.
      Help me~ 😀

    • #97777
      mith
      Participant

      what’s your cell type?

    • #97779
      Sohye
      Participant
      quote mith:

      what’s your cell type?

      The cell type is HeLa.
      Should I increase the time of trypsin with EDTA treatment more than 2min ?

    • #97785
      MrMistery
      Participant

      can’t hurt to try. Also try gently tapping the flask after the digest, that used to work well when I did tissue culture (never used HeLa cells though)

    • #97807
      Sohye
      Participant

      But..It’s not a flask. It’s a kind of chip made of PDMS. So does the tapping work well in case of chip? It’s too small…

    • #97835
      mith
      Participant

      I’ve tried trypsin up to 20 mins with both mcf7 and rex cartilage cells without much problems, try incubating for longer(@37 C). If too many die you’ll have to find another way.

You must be logged in to reply to this topic.

Members