cell culture on PDMS - Biology Forum

last updated by

mith 14 years, 10 months ago

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- February 20, 2010 at 6:08 pm #12798
  Sohye
  Participant
  How can I detach cells from surface of PDMS?
  I did trypsin with EDTA treatment for two minutes but, it didn’t work well.
  Help me~ 😀
- February 21, 2010 at 4:58 am #97777
  mith
  Participant
  what’s your cell type?
- February 21, 2010 at 5:42 am #97779
  Sohye
  Participant
  quote mith:
  what’s your cell type?
  The cell type is HeLa.
  Should I increase the time of trypsin with EDTA treatment more than 2min ?
- February 21, 2010 at 4:57 pm #97785
  MrMistery
  Participant
  can’t hurt to try. Also try gently tapping the flask after the digest, that used to work well when I did tissue culture (never used HeLa cells though)
- February 22, 2010 at 2:39 am #97807
  Sohye
  Participant
  But..It’s not a flask. It’s a kind of chip made of PDMS. So does the tapping work well in case of chip? It’s too small…
- February 23, 2010 at 4:13 am #97835
  mith
  Participant
  I’ve tried trypsin up to 20 mins with both mcf7 and rex cartilage cells without much problems, try incubating for longer(@37 C). If too many die you’ll have to find another way.
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