cell culture on PDMS

[Sohye]

How can I detach cells from surface of PDMS?
I did trypsin with EDTA treatment for two minutes but, it didn’t work well.
Help me~ 😀

[mith]

what’s your cell type?

[Sohye]

quote mith:

what’s your cell type?

The cell type is HeLa.
Should I increase the time of trypsin with EDTA treatment more than 2min ?

[MrMistery]

can’t hurt to try. Also try gently tapping the flask after the digest, that used to work well when I did tissue culture (never used HeLa cells though)

[Sohye]

But..It’s not a flask. It’s a kind of chip made of PDMS. So does the tapping work well in case of chip? It’s too small…

[mith]

I’ve tried trypsin up to 20 mins with both mcf7 and rex cartilage cells without much problems, try incubating for longer(@37 C). If too many die you’ll have to find another way.

[Author]

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