Change for plant expression

June 14, 2007 at 8:35 pm #7821

Participant

Hi every one
Can, if so how this plasmid be changed so the gene can be expressed in 1 small animals, 2 plants, 3 insects?

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June 14, 2007 at 9:14 pm #73822

LilKim

Participant

change the regulation region… to a promoter sequence that is only active in (unique-to) small animals, plants or insects …

not sure if there is a simpler solution?

June 14, 2007 at 10:04 pm #73824

mehdi71000

Participant

Thanks mate is there a way you can guide me. like what enzymes do I need and when and how to use them?

June 14, 2007 at 10:45 pm #73825

david23

Participant

u need a bunch restriction enzymes, and u need the exact sequence of the plasmid for the restriction sites. Go with eukaryote plasmids.

June 15, 2007 at 1:54 am #73832

canalon

Participant

Yep, in fact it is more complicated than just changing the promoter, because if you want to transfer your plasmid in a eucaryote you also need to insert an eucaryotic origin of replication, otherwise it will not be able to be replicated.

What you want might be what is called a shuttle plasmid, one that contains both origin of replication, and usually a promoter only for the final host (it is easy to transfer modify and propagate the plasmid in bacteria and then you can transfer it in plant/insect/mammalian cell when you want to express your gene.

June 15, 2007 at 2:58 pm #73837

mehdi71000

Participant

Thanks guys. I found this site it makes custom Dna
They have a software that allows you to make a vector and they will make it for you afterwards but I bet its going to be very expensive. Here is the link . http://www.dna20.com/genedesigner/GD_download.html

June 15, 2007 at 5:18 pm #73838

mehdi71000

Participant

Is this plasmid any good for plants (pblu)?
Thanks

One more thing.
Can I remove the gene from ecoli that is responsible for the translation in ecoli and add that to the plant so the plant can translate the plasmid?

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