Biology Forum Genetics Change for plant expression

last updated by mehdi71000 17 years ago
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    • #7821
      mehdi71000
      Participant

      Hi every one
      Can, if so how this plasmid be changed so the gene can be expressed in 1 small animals, 2 plants, 3 insects?


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    • #73822
      LilKim
      Participant

      change the regulation region… to a promoter sequence that is only active in (unique-to) small animals, plants or insects …

      not sure if there is a simpler solution?

    • #73824
      mehdi71000
      Participant

      Thanks mate is there a way you can guide me. like what enzymes do I need and when and how to use them?

    • #73825
      david23
      Participant

      u need a bunch restriction enzymes, and u need the exact sequence of the plasmid for the restriction sites. Go with eukaryote plasmids.

    • #73832
      canalon
      Participant

      Yep, in fact it is more complicated than just changing the promoter, because if you want to transfer your plasmid in a eucaryote you also need to insert an eucaryotic origin of replication, otherwise it will not be able to be replicated.

      What you want might be what is called a shuttle plasmid, one that contains both origin of replication, and usually a promoter only for the final host (it is easy to transfer modify and propagate the plasmid in bacteria and then you can transfer it in plant/insect/mammalian cell when you want to express your gene.

    • #73837
      mehdi71000
      Participant

      Thanks guys. I found this site it makes custom Dna
      They have a software that allows you to make a vector and they will make it for you afterwards but I bet its going to be very expensive. Here is the link . http://www.dna20.com/genedesigner/GD_download.html

    • #73838
      mehdi71000
      Participant

      Is this plasmid any good for plants (pblu)?
      Thanks

      One more thing.
      Can I remove the gene from ecoli that is responsible for the translation in ecoli and add that to the plant so the plant can translate the plasmid?


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