Cloning vector - Biology Forum

last updated by

MrMistery 16 years ago

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3 replies

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- April 4, 2008 at 2:21 am #9399
  roniadam
  Participant
  Hi everyone:
  how many enzymes are required to open a cloning vector for insertion of the cloned gene? I am using PFastBac vector and I cut it by one enzyme only. Is this will allow the interested fragment to insert into the vector or I have to use two enzymes???
- April 4, 2008 at 9:33 pm #83219
  canalon
  Participant
  1 enzyme is enough but the risk is religation of the vector without integration of the fragment. And of course you wil not be able to select the orientation of your insert, which can be a problem if you plan to express a protein.
- December 4, 2008 at 6:35 am #87551
  camzie
  Participant
  i have a question:
  Why isn’t the plasmid that contains the strongest promoter always the best expression vectors?
- December 10, 2008 at 3:57 am #87708
  MrMistery
  Participant
  you might want to turn the expression on and off using an inducer…
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