Biology Forum › Molecular Biology › Cloninig methodology for Recombinant L-asparaginase II
- AuthorPosts
- October 3, 2011 at 3:54 am #15455
vmadhukarParticipantDear friends
Iam working on making an e.coli based clone for producing L-asparaginase using r-DNA technology.
Iam succusfull in making the gene & the vector. But the expression levels are good at 0.6 -1.0 O.D ONLY.
Can anyone suggest me for a better vector & host for expressing the same.
Maddy - October 3, 2011 at 8:25 am #106606JackBeanParticipant
we can hardly suggest something better, if we don’t know current status. Are there any articles describing cloning of that gene?
- October 7, 2011 at 7:38 pm #106701CatParticipant
"But the expression levels are good at 0.6 -1.0 O.D ONLY."
This is common and fairly normal. Why is it a problem for you?
- AuthorPosts
You must be logged in to reply to this topic.
