Biology Forum › Molecular Biology › Cloninig methodology for Recombinant L-asparaginase II

last updated by Cat 12 years, 7 months ago
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    • October 3, 2011 at 3:54 am #15455
      vmadhukar
      Participant

      Dear friends
      Iam working on making an e.coli based clone for producing L-asparaginase using r-DNA technology.
      Iam succusfull in making the gene & the vector. But the expression levels are good at 0.6 -1.0 O.D ONLY.
      Can anyone suggest me for a better vector & host for expressing the same.
      Maddy

    • October 3, 2011 at 8:25 am #106606
      JackBean
      Participant

      we can hardly suggest something better, if we don’t know current status. Are there any articles describing cloning of that gene?

    • October 7, 2011 at 7:38 pm #106701
      Cat
      Participant

      "But the expression levels are good at 0.6 -1.0 O.D ONLY."

      This is common and fairly normal. Why is it a problem for you?

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