clonning - Biology Forum

last updated by

canalon 18 years, 2 months ago

3 voices

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- September 27, 2006 at 4:15 pm #5827
  kabuto
  Participant
  1. does digested clonning vector need to be purified?
  2. why only onion genome with fragment size 1-3kb used to build genomic library?
- September 27, 2006 at 5:37 pm #55482
  oppox
  Participant
  1. well I guess the enzymes could cut again if u dont, I dont remember how I did. Some enzymes can be stopped by for an example heating. you do best to download the manual to your enzymes
- September 28, 2006 at 12:38 pm #55569
  canalon
  Participant
  1-It is better as said by oppox, but sometimes heat inactivation is used followed by ligation in order to avoid loss of material during purification
  2- The longer the fragment, the harder to insert it into a vector in a stable manner.
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