Viewing 0 reply threads
  • Author
    • #9513

      I understand that 99% of the RNA isolated with my total RNA isolation kit (qbio problu kit) is rRNA. I am going to quantitate mRNA for specific genes and compare to 16S expression. Do my 16S primers amplify only mRNA for 16S or also all the rRNA? Stupid question maybe but I am confused on some recent studies that indicate over 1000x relative expression to the 16S which should be impossible if rRNA is amplified??

      for example:

      and … /153/1/102

      thank you

Viewing 0 reply threads
  • You must be logged in to reply to this topic.