Biology Forum › Cell Biology › decomplemeted serum
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- August 9, 2010 at 9:52 am #13637roxanalibeParticipant
Hi everybody,
I need to grow my cells in serum without any cytokine that comes in the serum. So, I need to eliminate the traces of cytokines from fetal bovine serum OR I need to find a commercial serum that has no cytokines. How can I do that? One idea could be that serum inactivation at 56°C for 30min could obtain this cytokine-depleted serum, but I am not convinced that this is the way.
Thank you for your input,
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