diluting primers - Biology Forum

last updated by

blcr11 17 years, 1 month ago

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- October 23, 2007 at 3:38 pm #8435
  elle1221
  Participant
  How much water should I add to dilute primer stocks (I’m guessing 100 μM) to 4 μM?
- October 23, 2007 at 3:40 pm #77140
  elle1221
  Participant
  I’m guessing it’s just 4 μl the stock to 96 μl H20, but I’d like to be sure.
- October 23, 2007 at 5:56 pm #77155
  blcr11
  Participant
  That should work, assuming the stock concentration really is 100 micromolar and I have no reason to believe otherwise.
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