DNA denautration by pH - Biology Forum

last updated by

kiekyon 18 years, 2 months ago

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- September 18, 2006 at 4:17 pm #5737
  princess.lauren
  Participant
  Hi!
  during my bio lecture (2nd yr) the prof mentioned that denaturation of DNA can occur by increasing temp or increasing pH. I understand that at higher temps the Hbonds will break between nucleotides, but how does denaturation occur at higher pH? i guess im looking for the process.
  thanks
  lauren
- September 19, 2006 at 6:55 pm #55048
  zami’87.
  Participant
  Adenine becomes soluble in weak acid…and uracil(speaking of RNA) in ammonia
- September 20, 2006 at 2:33 am #55071
  kiekyon
  Participant
  quote princess.lauren:
  Hi!
  during my bio lecture (2nd yr) the prof mentioned that denaturation of DNA can occur by increasing temp or increasing pH. I understand that at higher temps the Hbonds will break between nucleotides, but how does denaturation occur at higher pH? i guess im looking for the process.
  thanks
  lauren
  heat, high pH, low pH, they all break the H bond
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