DNA ligases - Biology Forum

last updated by

Sepals 15 years, 8 months ago

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- March 27, 2009 at 3:40 am #11125
  SysBio
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  After you "cut" DNA fragments using restriction enzymes, and then are "pasting" them together using DNA ligase…
  do you need to select a specific DNA ligase that matches with the specific restriction enzyme that was used?
- March 27, 2009 at 11:44 am #89886
  Sepals
  Participant
  No. It’ll anneal any broken strand. The vector is prevented from self-ligation by alkaline phosphatase.
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