Biology Forum Molecular Biology DNA ligases

last updated by Sepals 15 years ago
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    • #11125
      SysBio
      Participant

      After you "cut" DNA fragments using restriction enzymes, and then are "pasting" them together using DNA ligase…

      do you need to select a specific DNA ligase that matches with the specific restriction enzyme that was used?

    • #89886
      Sepals
      Participant

      No. It’ll anneal any broken strand. The vector is prevented from self-ligation by alkaline phosphatase.

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