I’m new here so I’m hoping I’m doing this correctly! I am a sophmore at a small private university and am studying cell biology. We just completed a lab on plasmid DNA and ran an agarose gel producing two bands. My professor wants to know why there are two bands of undigested PBR322 (I assume the plasmid?) when the DNA is circular.
I know a few things already:
The smaller the particle the faster it moves through the gel.
Supercoiled DNA moves the fastest so chances are its the lower bar.
We did not cut the DNA so we can not assume size difference.
Could anyone help me figure out what the other piece of DNA could be?
Thanks so much.
Well, a topoisomers are varying forms of closed circular dna molecules. So, are you saying (or should i say pointing me to the fact) that the other band of DNA is a topoisomers? Sorry if I sound slow at this…its kind of a first time for me having to explain what I’m doing in lab.