Doubling time for cells

[kpatel]

Can anyone help me out as how to find the doubling time of a particular cell line?

Thanks.

[LilKim]

I don’t know where you can "look-up" that type of info.

But, it’s pretty easyy to figure it out.

You’ll just have to have a way of counting the cells… and then re-counting them every hour for like… 8-10 hours. And you should be able to get an approximate time.

[MrMistery]

What species are you working with? Is it some bacteria? Because some bacterial species have been studied and the average generation time published.

[kpatel]

It is a rat Schwann cell line I am working with.

[MrMistery]

You could try finding it in the literature, but i doubt you would have much luck. But you could determine it experimentally…

[Priscilla]

1. count the cells when it’s confluent.(call it A)
2. split. and put a certain amount in each flask.(call it B)
3. After a few days, count the cells again.(call it C)(Record also the number of days. call it D)
B x 2to the power N = C
Solve for N. N is the number of doubles.
So it takes D days to have N number of doubles)
If it takes you 3 days to have 5 doubles, 1 double will take you 3/5 days. Which is 0.6day
0.6 day will be the doubling time.

[snoopline]

Doubling time computing is correct only in exponential growing phase.
It could be hard to get a reliable calculus with adherent cells compare to suspension cells.
Split your cells, measure their concentration : c1
Let them grow during h hours
Count them again : c2

The doubling time(in hours)=h*ln(2)/ln(c2/c1)

You can use decimal or neperien logarithm.
You can use this formula to forcast the cell expension or to calculate the volume to split to get a target concentration after a period of growing.

A software do this for you at http://www.doubling-time.com

For a given cell lines, the doubling time changes regarding the number of passages, the serum lot, the way you pass them…
For cell line info, check on : http://www.dsmz.de/human_and_animal_cell_lines/cell_line_index.php

[snoopline]

You can use the online calculator to calculate the doubling time of your cells.
http://www.doubling-time.com/compute.php?lang=en
here is the chinese version !
http://www.doubling-time.com/compute.php?lang=zh

Very convenient if you have several time points.

[Adah]

Hello, does anyone here can explain to me what is the information can we get from the cell doubling time t..is it telling us a measurement/or indicator ?…say: t measure a successful of radiation treatment? .i am not in biological area, i am a mathematics student. So can anyone here could explain to me please…..

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[JackBean]

well, it can tell you, how preferable is that environment 😉

[Author]

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