I don’t know where you can "look-up" that type of info.

But, it’s pretty easyy to figure it out.

You’ll just have to have a way of counting the cells… and then re-counting them every hour for like… 8-10 hours. And you should be able to get an approximate time.

What species are you working with? Is it some bacteria? Because some bacterial species have been studied and the average generation time published.

You could try finding it in the literature, but i doubt you would have much luck. But you could determine it experimentally…

1. count the cells when it’s confluent.(call it A)
2. split. and put a certain amount in each flask.(call it B)
3. After a few days, count the cells again.(call it C)(Record also the number of days. call it D)
B x 2to the power N = C
Solve for N. N is the number of doubles.
So it takes D days to have N number of doubles)
If it takes you 3 days to have 5 doubles, 1 double will take you 3/5 days. Which is 0.6day
0.6 day will be the doubling time.

Doubling time computing is correct only in exponential growing phase.
It could be hard to get a reliable calculus with adherent cells compare to suspension cells.
Split your cells, measure their concentration : c1
Let them grow during h hours
Count them again : c2

The doubling time(in hours)=h*ln(2)/ln(c2/c1)

You can use decimal or neperien logarithm.
You can use this formula to forcast the cell expension or to calculate the volume to split to get a target concentration after a period of growing.

A software do this for you at http://www.doubling-time.com

For a given cell lines, the doubling time changes regarding the number of passages, the serum lot, the way you pass them…
For cell line info, check on : http://www.dsmz.de/human_and_animal_cell_lines/cell_line_index.php

You can use the online calculator to calculate the doubling time of your cells.
http://www.doubling-time.com/compute.php?lang=en
here is the chinese version !
http://www.doubling-time.com/compute.php?lang=zh

Very convenient if you have several time points.

Hello, does anyone here can explain to me what is the information can we get from the cell doubling time t..is it telling us a measurement/or indicator ?…say: t measure a successful of radiation treatment? .i am not in biological area, i am a mathematics student. So can anyone here could explain to me please…..

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well, it can tell you, how preferable is that environment 😉