Biology Forum Molecular Biology Electrophoresis conditions

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      genetherapy
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      Hi all,
      I use 35 centimetre agarose gel tank, we use constant watt (20 watt), change only the mA and voltage. I set voltage to 140V and mA to 60. Is it OK? Because I see vaporization above the tank. My samples seem bad on agarose when I look them at UV
      Thanks

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