Biology Forum Molecular Biology electrophoresis

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    • #5535
      kabuto
      Participant

      which one is better in gel electrophoresis??
      radioactive probe or non-radioactive probe
      why??

    • #53714
      mmiaosmiling
      Participant

      that will depend on what ur aim is and how accurate u need.

    • #58605
      kabuto
      Participant

      can u explain further? 😯

    • #58637
      canalon
      Participant

      You do not probe in electrophoresis… you use a probe in a southern. And then radioactivity for probe is almost religion 🙂
      More seriously some people will defend the non-radiaoctive probes as as good as radioactive, while others will tell you that radioactive is best. In my experience both have problems and at least non-radioactive are much less trouble to handle.

    • #58642
      sachin
      Participant

      Its best to use non radioactive probe. As intense care to handle and detect.

      But non-radioactive or say "fluroscent calating" is best practiced for AGEP, PGAL etc…

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