Biology Forum › Molecular Biology › electrophoresis
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August 21, 2006 at 1:46 pm #5535kabutoParticipant
which one is better in gel electrophoresis??
radioactive probe or non-radioactive probe
why?? -
August 21, 2006 at 1:59 pm #53714mmiaosmilingParticipant
that will depend on what ur aim is and how accurate u need.
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November 8, 2006 at 8:01 pm #58605kabutoParticipant
can u explain further? 😯
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November 9, 2006 at 2:57 am #58637canalonParticipant
You do not probe in electrophoresis… you use a probe in a southern. And then radioactivity for probe is almost religion 🙂
More seriously some people will defend the non-radiaoctive probes as as good as radioactive, while others will tell you that radioactive is best. In my experience both have problems and at least non-radioactive are much less trouble to handle. -
November 9, 2006 at 4:26 am #58642sachinParticipant
Its best to use non radioactive probe. As intense care to handle and detect.
But non-radioactive or say "fluroscent calating" is best practiced for AGEP, PGAL etc…
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