I know that a friend of mine happened to denaturate his protein when he tried to store it in a freezer, however it dont apply to all proteins. Taq polymerase for instanse is stored below zero, it comes from a heat resistent bacteria. Its hard to say if yours will denaturate.
yup, I think like that too. The reason I think is related to the secondary and tertiary structure of the enzyme, where hydrogen binding and hydrophobic interaction would not be same as the active enzyme (you’ll get this idea when you imagine how ice float in water 😆 )
But one question that still confusing me, I found that lipase is still active even in the temperature 0 ºC……:?
Many proteins can denature if the medium they are in freezes. Most enzymes used in molecular biology that are stored at -20 C are kept in a glycerol buffer that prevents freezing. You could try doing this. If you’d like to store your proteins frozen, you could try snap freezing them in liquid nitrogen. This process seems to cause less denaturation for many proteins.