Biology Forum Molecular Biology Enzyme from different species

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    • #12306

      Would enzymes from different species (fish and human) have same optimum temperature and why? I am guessing that they would have the same optimum temperature, but fish would likely be able to use enzyme at a lower temperature than humans.

    • #95061

      Not really, first of all, that depends on what fish you pick. If some from sea of "normal" temperature, than they can have similar temperature optimum, but if you pick some polar fish, than its protein will have definitelly lower T optimum and also will be more T stable, because the fish must live in cold enviroment and it can’t regulate its temperature as mammals can 😉

    • #95075

      just to add to the info
      plants have diff plants with diff enzymes working at diff optimum temp.
      the root enzymes work at temp lower than that of the shoot.
      what do you think jackbean?
      he is the botanist though.

    • #95077

      Why, the hell, did I tell you? 🙄 😆
      Actually, I don’t think so. I’m not so much experienced, but from my field of study, most of the enzymes are expressed through the whole plant (at different tissues or organs, but throughout whole plant, you can’t say, it’s strictly root enzyme).
      Regarding the temperature optimum, I don’t think, they have several forms differentiated by the T optima, it is probably usually lower, than 37°C, usually it’s a priori taken as 30°C, but e.g. I found with my enzyme, that althrough everybody said, it’s highly unstable, it’s T optima was about 40°C with 1 hour incubation and about 37°C with 3 hour incubation
      (previous reports said, that it’s inactivated after like 20 minutes @ 37°C)

    • #95109

      Okay, so the same enzyme in a different species would have a different optimum temperature because of differentiated forms of that enzyme?

    • #95112

      Probably yes

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