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    • #9165
      az
      Participant

      Greetings

      I need coding sequences of eukaryotes. NCBI ftp contains both frn and ffn files. Can you, please, tell me, what is the difference, and what format I should use for the analysis (it should be cDNAs only)

    • #82237
      mith
      Participant

      there’s a readme file in each folder. I suggest you read it.

      The files are present in the following formats:

      *.asn = ASN.1 file, print form, replaces .prt
      *.faa = FASTA Amino Acid file
      *.ffn = FASTA nucleotide coding regions file
      *.fna = FASTA Nucleic Acid file
      *.gbk = GenBank flat file format
      *.ptt = Protein Table
      *.val = ASN.1 binary format
      *.tar.Z = unix tar and compressed files (not all files are compressed),
      where all of the above files are present. If you want all
      files for a group, all you need is to FTP this one file.

    • #82242
      az
      Participant

      Thanks, but FYI, not all directories in NCBI FTP site contain readme file. That was the reason, I asked for advice.

    • #82257
      mith
      Participant

      my apologies.

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