Fungal DNA extrtaction problem

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    • #10863
      shin2
      Participant

      Dear all ❓
      I am trying to extract DNA (size >30kb) from a filamentous fungi. no mater what technique i used the DNA size is always less then 15 kb. I will be thankful if any one can help me and send me a protocol that is capable of extracting greater then 30 kb fragment DNA

      Thank you

    • #89115
      domwood
      Participant

      I’m probably totally wrong here, but how much do you know about the fungal DNA. 15kb piece seems to me to be coincidentally 50% of 30kb. Is it possible the the DNA in the fungus is two separate chromosomes?

    • #89152
      shin2
      Participant

      I am really sorry i did not pick your point, if you could explain clearly? I have compared my extracted DNA with Limbda DNA (Hind III digested) and 1 kb DNA ladder. all the time its greater then 10 kb band and less then 24 kb of Limbda DNA.

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