Fungal DNA extrtaction problem - Biology Forum

last updated by

shin2 15 years, 9 months ago

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- February 7, 2009 at 8:44 am #10863
  shin2
  Participant
  Dear all ❓
  I am trying to extract DNA (size >30kb) from a filamentous fungi. no mater what technique i used the DNA size is always less then 15 kb. I will be thankful if any one can help me and send me a protocol that is capable of extracting greater then 30 kb fragment DNA
  Thank you
- February 15, 2009 at 3:11 pm #89115
  domwood
  Participant
  I’m probably totally wrong here, but how much do you know about the fungal DNA. 15kb piece seems to me to be coincidentally 50% of 30kb. Is it possible the the DNA in the fungus is two separate chromosomes?
- February 16, 2009 at 2:32 pm #89152
  shin2
  Participant
  I am really sorry i did not pick your point, if you could explain clearly? I have compared my extracted DNA with Limbda DNA (Hind III digested) and 1 kb DNA ladder. all the time its greater then 10 kb band and less then 24 kb of Limbda DNA.
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