Hi, I am doing a science fair project, and there is a lot of background information that I need to understand before doing the project. I’m wondering if anyone knows any useful websites or if anyone can explain certain concepts to me. The objective of my project is to create a GFP-Protein G chimeric protein for immunological applications. I have already done some research on how PCR (polymerase chain reaction) works. However, I still do not understand the following things:

– Protein G genes
– Recombinant His-tagged proteins (and how they are purified by affinity chromatography on a nickel-agarose column)

Thank you very much for your help!

– Tim