Biology Forum › Molecular Biology › gel
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- January 25, 2006 at 10:58 am #3332
ShirleyParticipantI have made some competent cells and transform them with a vector. Then I did a plasmid isolation. The DNA concentration is 100ug/ml. I run a gel but no band is detected. So you think my competent cells are really competent?
- January 25, 2006 at 3:23 pm #38419canalonParticipant
There are plenty of things that may have gone wrong:
– competent cells as you said,
but also :
– plasmid isolation
– Selection of transformantsWithout more details I can’t help.
- January 26, 2006 at 7:20 am #38508sdekivitParticipantquote Canalon:There are plenty of things that may have gone wrong:
– competent cells as you said,
but also :
– plasmid isolation
– Selection of transformantsWithout more details I can’t help.
or the gel isn’t made properly
- January 26, 2006 at 3:04 pm #38552canalonParticipantquote sdekivit:quote Canalon:There are plenty of things that may have gone wrong:
– competent cells as you said,
but also :
– plasmid isolation
– Selection of transformantsWithout more details I can’t help.
or the gel isn’t made properly
True enough.
Run in water? (I did that once, it melted 😥 )
Forget to stain?
Did not load? I once had a problem with a loading buffer that wasn’t dense enough to make the solution go into the gel. It would just stay on top and leak out 👿 )
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