Biology Forum › Molecular Biology › glass vs. plastic when working with lipids
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- November 28, 2007 at 8:59 pm #8673
unibasParticipantHi,
I need to analyze the fat content of stool samples from mice. I found a protocol that involves chloroform:methanol extraction and weighing the extracted fat.
The entire protocol is with glass tubes, but we don’t have a centrifuge that fits glass tubes. How important is it to use glass and not plastic? I anyway can’t use glass, so is it worth bothering with this protocol?Thanks!
- November 28, 2007 at 9:22 pm #78666MrMisteryParticipant
i don’t see how glass/plastic tubes would make a significant difference. But my lab experience is extremely limited.
- November 28, 2007 at 9:27 pm #78668unibasParticipant
I suppose lipids stick to plastic. I think most of the protocols for lipid analysis ask for glass tubes.
- November 28, 2007 at 9:30 pm #78669MrMisteryParticipant
they do stick to plastic, but if they are in a methanol chloroform solution i doubt they do
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