Biology Forum Molecular Biology Help! Convert mg/ml to mg/kg

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6 replies
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• #14019
Participant

Okay, I have nitroglycerin at 5mg/ml (in liquid form).
My mice need a dose of 10mg/kg.
I have 8 mice weighing approximately 30g.
How do I figure out how to dilute to the proper concentration and volume?
I think I need to use C1V1=C2V2, but I don’t know how to get the end result.

• #102013
canalon
Participant

You do not need C1V1=C2V2.
Your mice need 10 mg/kg and weight 0.03 kg, calculate X (in mg) the total amount to inject.
You have a 5 mg/ml solution divide x by 5 and you know Y the number of ml you need to inject.

• #102017
Participant

I am still confused….I can use c1v1=c2v2…but I guess what I really need to know is how I get C2?
With a C2 of 1mg/ml, and If I am injecting .3ml for a .03kg mouse, then how did I get to this point?
So….
For 8 mice weighing approx 30g each (+a little extra just in case) I was given this information.
C1= 5mg/ml
V1= .8ml
C2= 1mg/ml
V2=4
I need to add saline to the nitroglycerin to get the proper concentration, dose and volume. I’m lost…

• #102018
canalon
Participant

OK the complete solution for a 30g mouse you need
0.03kg x 10 mg/kg = 0.3mg per mouse
so with your initial solution you are suppose to inject 0.06ml or 60µl.
If you prepare a 1mg/ml solution, 300µl are necessary

• #102019
Participant

Ok now that totally makes sense! Thank you so much for your help, I know i must seem absolutely retarded. I swear I am not!

One last question. How would I prepare the 1mg/ml solution with the original concentration that i have which is 5mg/ml nitrolgycerin?

and I also understand how you got to .3mg per mouse, but how did you convert that to 300microliters per mouse? Because that makes sense, we inject .3ml for a 30g mouse.

Thanks you again, i dont know why i cant wrap my head around this

• #102026
canalon
Participant

1/ dilute 5 times, or in this case use the c1v1=c2v2
2/ 1mg/ml means that you have 1mg of product in 1ml of solution. if you want 0.3 mg of product, how much solution?

PS: brain usage is not optional.

• #220053
cake55
Participant

maybe I am pretty late lol, but it would be useful for all forum users – there is a free ml to l converter which is super handy for such tasks.

You’re welcome

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