Hi there,

On friday (2nd sep) I carried out a single colony PCR and streaked a new plate with the colonies I selected…I kept it overnight and put the plate in the fridge sat morn…yesterday (monday) I did an overnight culture on one of the colonies and today made a glycerol stock of it…but I I got home and realised I made a mistake and plan to do another O/N culture tomorrow (thursday) and new glycerol stock (Friday)..is this ok..if i pick a colony of the streaked plate will it still be ok..I was told it was better to make a glycerol stock asap and read that 2-3 weeks is ok..so just wondering is less than a week alright?

NB: I used lactococcus Lactis