How to calculate mass of target Dna in ng - Biology Forum

last updated by

genetherapy 12 years, 4 months ago

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- July 18, 2012 at 7:37 am #16683
  siddharthsameer
  Participant
  hello Everyone
  I have a small question, how do I calculate the mass of target DNA in ng as I am looking to calculate the amount of Restriction enzyme required for the digestion of my target DNA.Or How should I calculate the Volume needed for Restriction enzyme to digest my Vector And Target Dna.
  I have only the concentartion of my Target DNA AND Vector and as well as the base pair. are these information sufficient to calculate the volume or do I need tio have more information for the calculation.
  Kindly help me I am stuck with this problem..
  with regards
- July 30, 2012 at 11:25 am #111974
  genetherapy
  Participant
  Look at this web-sites,the informations that they have will help you
  http://www.molecularstation.com/dna/res … digestion/
  http://www.methodbook.net/dna/restrdig.html
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