Biology Forum Molecular Biology How to calculate mass of target Dna in ng

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    • #16683
      siddharthsameer
      Participant

      hello Everyone
      I have a small question, how do I calculate the mass of target DNA in ng as I am looking to calculate the amount of Restriction enzyme required for the digestion of my target DNA.Or How should I calculate the Volume needed for Restriction enzyme to digest my Vector And Target Dna.
      I have only the concentartion of my Target DNA AND Vector and as well as the base pair. are these information sufficient to calculate the volume or do I need tio have more information for the calculation.
      Kindly help me I am stuck with this problem..
      with regards

    • #111974
      genetherapy
      Participant

      Look at this web-sites,the informations that they have will help you

      http://www.molecularstation.com/dna/res … digestion/
      http://www.methodbook.net/dna/restrdig.html

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