Biology Forum Molecular Biology How to know how to do the electroporation?

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    • #9130

      Hi! Does anyone know how I can find out what parameters that is good to use for my cell line (PLC/PRF/5) Have tried to google it for a while but do not find anything. Thanks!

    • #82052

      It’s an old paper, but it’s electroporation into hepatocytes anyway. … obtype=pdf

      Also old, but maybe useful:
      “Animal Cell Electroporation and Electrofusion Protocols.” Methods in Molecular Biology, Vol 48, Humana Press, 1995

      You might take a look at some of the papers using dendrimeric polymers to enhance transfection efficiency. A few people are using them in combination with electroporation, but I don’t do these kinds of things so I can’t say very much about their efficacy. I’m strictly a Ca-induced-transfections-into-bacteria kind of person, although I know that the same (Ca-induced) method works for fibroblasts, at least.

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