How to know how to do the electroporation? - Biology Forum

last updated by

blcr11 16 years, 10 months ago

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- February 18, 2008 at 3:09 pm #9130
  Ammie
  Participant
  Hi! Does anyone know how I can find out what parameters that is good to use for my cell line (PLC/PRF/5) Have tried to google it for a while but do not find anything. Thanks!
- February 20, 2008 at 10:53 pm #82052
  blcr11
  Participant
  It’s an old paper, but it’s electroporation into hepatocytes anyway.
  http://www.pubmedcentral.nih.gov/picren … obtype=pdf
  Also old, but maybe useful:
  “Animal Cell Electroporation and Electrofusion Protocols.” Methods in Molecular Biology, Vol 48, Humana Press, 1995
  You might take a look at some of the papers using dendrimeric polymers to enhance transfection efficiency. A few people are using them in combination with electroporation, but I don’t do these kinds of things so I can’t say very much about their efficacy. I’m strictly a Ca-induced-transfections-into-bacteria kind of person, although I know that the same (Ca-induced) method works for fibroblasts, at least.
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