Hyperchromicity of DNA - Biology Forum

last updated by

klou 14 years, 12 months ago

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- November 27, 2009 at 6:14 pm #12363
  klou
  Participant
  In an experiment to determine the Hyperchromicity of DNA, the absorbance value for undenatured DNA was 0.390 and the value for heat- denatured DNA was 0.790. Calculating the percentage increase gave me a 102% increase, where it should have been around 40-50%! What possible reasons could there be for this large increase?
- November 28, 2009 at 12:12 am #95413
  JackBean
  Participant
  I think, you have swithed the values, didn’t you?
- November 28, 2009 at 4:08 pm #95450
  klou
  Participant
  quote JackBean:
  I think, you have swithed the values, didn’t you?
  I’m not sure what you mean.
  The calculation for the hyperchromicity is (A260 denatured – A260 control) / A260 control x 100
  Which gives 102%
- November 29, 2009 at 1:43 am #95472
  JackBean
  Participant
  quote klou:
  the absorbance value for undenatured DNA was 0.790 and the value for heat- denatured DNA was 0.390.
  I think this. I think, that the denatured DNA has higher absorbance, doesn’t it?
- November 29, 2009 at 1:02 pm #95500
  klou
  Participant
  quote JackBean:
  quote klou:
  the absorbance value for undenatured DNA was 0.790 and the value for heat- denatured DNA was 0.390.
  I think this. I think, that the denatured DNA has higher absorbance, doesn’t it?
  I got it the wrong way round in my first post. But I did the calculation correctly, so that doesn’t change the result.
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