Biology Forum Microbiology Identifying an unknown

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      Hi, I am currently taking my microbiology lab final and as is with most microbiology classes, the final consists of identifying an unknown. In my case, we were all given a different test tube which contains two different bacteria. I was hoping to find some help from you all.
      So far, I have done a gram’s stain, acid-fast stain and a spore stain.
      The gram’s stain concluded many purple rod-shaped cells, which would lead me to believe that it is gram-positive.
      The acid-fast stain concluded both pink and purple colors
      The spore stain did not yield any results.

      On top of the stains, I have done dishes of a variety of media.
      MSA: I attached a picture of this to my post, as it had the most growth. In case you can’t tell, it is white, flaky, and milky looking.
      TSA: Yellow colones
      NA: White colonies
      EMB: No growth at all

      The possible bacteria that we can be given are: Bacillus subtillis, Staph aureus, Mycobacterium Smegmatis, Micrococcus Luteus, Proteus Vulgaris, Serratia mercescens, Pseudomonas aeruginosa, Escherichia coli
      Also, Staph and micrococcus are not a possible combination.

      I’m fairly certain that one of them is Mycobacterium Smegmatis.

      I really appreciate the help!


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