Biology Forum Molecular Biology In- gel rehydration

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      proteinman
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      Hello everyone,

      Can anyone tell me please is there a method of in gel rehydration without making fresh reagents (rehydration buffers). I have a 110mm long IPG strip and I know, that there is a way to rehydrate it with my cell lysate, adding two buffers (I don’t know its consistention).

      Thank you in adwance,

      Andrius

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