Biology Forum Molecular Biology Insulin questions

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    • #4554
      G Campbell
      Participant

      What are the enzymes that are used for cutting Insulin? Am I right in thinking that EcoR1, BamH1, and Hind111 are the ones that cut it and what is the vector that can be used for Insulin?

    • #47080
      angel
      Participant

      Insulin is a protein . How can restriction endonucleases cut it?
      Are you refering to insulin Gene???

    • #47113
      G Campbell
      Participant

      yeah i am refering to the gene

    • #47180
      d00d
      Participant

      yeah, those are valid restriction enzymes.
      the vector is a plasmid (or circular DNA) obtained from a bacterium.

    • #47578
      G Campbell
      Participant

      Could someone give me any good sites that explain about inserting a vector into E.coli? I have looked but nothing good enough seems to come up.

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