Biology Forum › Molecular Biology › Insulin questions

last updated by G Campbell 18 years ago
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    • April 22, 2006 at 10:36 am #4554
      G Campbell
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      What are the enzymes that are used for cutting Insulin? Am I right in thinking that EcoR1, BamH1, and Hind111 are the ones that cut it and what is the vector that can be used for Insulin?

    • April 22, 2006 at 7:28 pm #47080
      angel
      Participant

      Insulin is a protein . How can restriction endonucleases cut it?
      Are you refering to insulin Gene???

    • April 23, 2006 at 11:47 am #47113
      G Campbell
      Participant

      yeah i am refering to the gene

    • April 24, 2006 at 1:49 am #47180
      d00d
      Participant

      yeah, those are valid restriction enzymes.
      the vector is a plasmid (or circular DNA) obtained from a bacterium.

    • April 28, 2006 at 8:21 pm #47578
      G Campbell
      Participant

      Could someone give me any good sites that explain about inserting a vector into E.coli? I have looked but nothing good enough seems to come up.

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