Insulin questions - Biology Forum

last updated by

G Campbell 18 years, 7 months ago

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- April 22, 2006 at 10:36 am #4554
  G Campbell
  Participant
  What are the enzymes that are used for cutting Insulin? Am I right in thinking that EcoR1, BamH1, and Hind111 are the ones that cut it and what is the vector that can be used for Insulin?
- April 22, 2006 at 7:28 pm #47080
  angel
  Participant
  Insulin is a protein . How can restriction endonucleases cut it?
  Are you refering to insulin Gene???
- April 23, 2006 at 11:47 am #47113
  G Campbell
  Participant
  yeah i am refering to the gene
- April 24, 2006 at 1:49 am #47180
  d00d
  Participant
  yeah, those are valid restriction enzymes.
  the vector is a plasmid (or circular DNA) obtained from a bacterium.
- April 28, 2006 at 8:21 pm #47578
  G Campbell
  Participant
  Could someone give me any good sites that explain about inserting a vector into E.coli? I have looked but nothing good enough seems to come up.
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