Biology Forum Molecular Biology Integrating plasmid to Yeast

2 voices
2 replies
  • Author
    Posts
    • #13178
      molecule
      Participant

      Hi, I;m not familiar with this technique and was wondering if someone can help out. I have a plasmid that has an ARS and my gene of interest and I need put this gene of interest in to an yeast strain to study it. Does anyone know how I can do that. More details about the technique will be very helpful. I tried to read about some yeast integration it was hard to comprehend.
      thanks

    • #99205
      JackBean
      Participant

      what yeast do you use? I have used pPICZ and pGAPZ in Pichia, there was part of one endogenous genes, the plasmid was cut in one place and incorporated by homologous recommbination

    • #99233
      molecule
      Participant

      I’m looking at Fission yeast. Its the procedeur as to how to do that is what I;m no familiar with. thanks

You must be logged in to reply to this topic.

Members