Integrating plasmid to Yeast - Biology Forum

last updated by

molecule 14 years, 8 months ago

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- April 22, 2010 at 5:07 am #13178
  molecule
  Participant
  Hi, I;m not familiar with this technique and was wondering if someone can help out. I have a plasmid that has an ARS and my gene of interest and I need put this gene of interest in to an yeast strain to study it. Does anyone know how I can do that. More details about the technique will be very helpful. I tried to read about some yeast integration it was hard to comprehend.
  thanks
- April 22, 2010 at 7:51 am #99205
  JackBean
  Participant
  what yeast do you use? I have used pPICZ and pGAPZ in Pichia, there was part of one endogenous genes, the plasmid was cut in one place and incorporated by homologous recommbination
- April 23, 2010 at 12:32 am #99233
  molecule
  Participant
  I’m looking at Fission yeast. Its the procedeur as to how to do that is what I;m no familiar with. thanks
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