Biology Forum › Cell Biology › iodine stain on potato cell

last updated by cbryant 13 years, 2 months ago
8 voices
14 replies
  • Author
    Posts
    • November 6, 2005 at 3:59 pm #2390
      xpeix
      Participant

      Hi,

      we recently did a lab where we stained the potato cell with iodine. For some reason, we observed more sacs in the cell after staining…just wondering if this is normal and if the “sacs” we sall might actually be just bubbles(didn’t look like bubbles though).

      -Thanks in advance

    • November 7, 2005 at 4:01 pm #32221
      Poison
      Participant

      iodine is the indicator of starch. and starch is a compound found in potato (actually in most of the plant cells).

    • November 7, 2005 at 6:46 pm #32234
      Starlight189
      Participant

      I did that experiment! Well I think it was that experiment…for some reason I couldn’t find any sacs at all!

    • November 8, 2005 at 2:38 am #32281
      Navin
      Participant

      Besides being an indicator of starch, iodine also acts as a stain and helps us to see certain features in more detail. However, ive never seen any “sacs” before. Could you post a photo?

    • November 8, 2005 at 3:26 am #32282
      Navin
      Participant

      I tried it myself and here are the results:
      (you can compare with mine)

      Attachments:

    • November 8, 2005 at 5:03 am #32285
      xpeix
      Participant

      Thanks for replies.

      After doing some research, I found that I was looking at amyloplasts. It basically looked like this (but darker, I put too much iodine 🙁 )

      The only thing that i don’t understand is why i saw more amyloplasts that are smaller after dropping iodine on the slide. (originally in tap water)

      please help

      -thanx

    • November 8, 2005 at 7:49 am #32289
      Navin
      Participant

      i guess the iodine stains it, making it more visible. I doubt any reactions took place to make more amyloplasts.

    • November 8, 2005 at 2:04 pm #32294
      chetanladdha
      Participant

      hey,, water potential = solute potential +pressure potential
      is ok,, but from where jelly babies r coming……

    • November 8, 2005 at 8:58 pm #32338
      MrMistery
      Participant

      I’m guessing that they are harder to stain and you only see them at a higher concentration of iodine. just an opinion

    • November 8, 2005 at 10:53 pm #32362
      Starlight189
      Participant
      quote chetanladdha:

      hey,, water potential = solute potential +pressure potential
      is ok,, but from where jelly babies r coming……

      Bassetts of course 🙄

    • November 9, 2005 at 6:51 pm #32442
      MrMistery
      Participant

      What does Bassetts mean? Sorry i am from Romania

    • November 9, 2005 at 10:22 pm #32469
      Starlight189
      Participant

      Tis ok, It’s a sweet company…no one gets my jokes :-(, I think I’ll change my signature lol.

    • November 10, 2005 at 1:01 am #32480
      xpeix
      Participant

      thanks everyone for all your replies, I guess I’ll just go with the theory that iodine made it more visible.

      if any1 has anymore suggestions, i would greatly appreciate it

      -xpeix

    • September 29, 2011 at 2:45 am #106486
      shivangimisra
      Participant

      Hi all,

      I have to do this experiment tommorow. I hope that its fun please let me know what you all think about this.

      Shivangi

    • October 11, 2011 at 3:48 am #106799
      cbryant
      Participant

      Iodine is a stain specific for starch, meaning when it comes in contact with starch containing substances, it changes colors.

  • Author
    Posts

You must be logged in to reply to this topic.

Related posts:

  1. water potential of sweet potato and ordianry potato??
  2. Iodine and plant cells
  3. Endospore stain
  4. Using oxidizing agents in gram stain

Members