Biology Forum › Microbiology › is this correct????
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- November 23, 2009 at 4:31 am #12335
Bill123ParticipantI am writing the thesis, anybody can tell me are there some mistake in the following sentence?? Thank you!!
The pUC19-p450 and pUC19-p450-bm3 plasmids containing p450 and p450-bm3 genes respectively were used as templates for PCR amplification using primers pYES-For (5’- ATGCATGCATGCATGCATGCATGC-3’) and pYES-Rev (5’- ATGCATGCATGCATGCATGCATGC-3’), both of which anneal to the pUC19 vector.
- November 23, 2009 at 9:35 am #95249JackBeanParticipant
Except, that both your primers seem tzo have the same sequence, it looks fine to me.
- November 23, 2009 at 9:11 pm #95258Bill123Participantquote JackBean:Except, that both your primers seem tzo have the same sequence, it looks fine to me.
😀 Thank you for your help!!
- November 26, 2009 at 6:22 pm #95365MrMisteryParticipant
the fact that both your primers are identical means you got something wrong
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