M13 vector

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Is it true that you can clone up to 500-600nt when you use M13 as a vector?

[wbla3335]

I don’t know what the largest insert on record is, but Maniatis says 40+ kilobases is possible. One nice thing about the M13 vectors is that they produce single-stranded DNA, which has provided the best quality sequence I have ever seen, even with 32P back in the old days.

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Thank you for the info, wbla3335

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