M13 vector - Biology Forum

last updated by

ping 17 years, 10 months ago

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- December 24, 2006 at 8:06 am #6635
  ping
  Participant
  Is it true that you can clone up to 500-600nt when you use M13 as a vector?
- December 27, 2006 at 11:49 am #64124
  wbla3335
  Participant
  I don’t know what the largest insert on record is, but Maniatis says 40+ kilobases is possible. One nice thing about the M13 vectors is that they produce single-stranded DNA, which has provided the best quality sequence I have ever seen, even with 32P back in the old days.
- December 27, 2006 at 12:33 pm #64125
  ping
  Participant
  Thank you for the info, wbla3335
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