Biology Forum › Molecular Biology › molecular biology

last updated by Helix 18 years ago
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    • April 5, 2006 at 11:06 am #4332
      Helix
      Participant

      Hi,
      does anyone know what is the maximum length of DNA that can be amplified by PCR? Please post the answer if some one knows.

    • April 10, 2006 at 8:56 am #45568
      angel
      Participant

      may be its 1000 bp. i am not sure

    • April 10, 2006 at 3:34 pm #45585
      canalon
      Participant

      More than that! I regularly amplify over 2 kb. with good conditions and enzymes, you can go way over that. Probably up to 10 kb.

    • April 10, 2006 at 4:36 pm #45590
      Trev
      Participant

      it all depends from polymerase and PCR-method. My scientific leader amplified more than 16 kb.

    • April 10, 2006 at 6:23 pm #45594
      LilKim
      Participant

      i’ve personally amplifed 8KB fragments. the problem is that regular taq has a high error rate (approx. 1 error/1000bp). Therefore, if fidelity is extremely important use hi-fi taq (hi fidelity taq)… which uses MgSO4 (instead of MgCL). Also.. the extension time has to be increased so that taq has enough time to extend….

    • April 11, 2006 at 6:56 am #45645
      Helix
      Participant

      My sincere thanks to all who have responded to my post. Your answers and suggestions will be very much helpful to me.

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