molecular biology - Biology Forum

last updated by

Helix 18 years, 7 months ago

5 voices

5 replies

Author
Posts
- April 5, 2006 at 11:06 am #4332
  Helix
  Participant
  Hi,
  does anyone know what is the maximum length of DNA that can be amplified by PCR? Please post the answer if some one knows.
- April 10, 2006 at 8:56 am #45568
  angel
  Participant
  may be its 1000 bp. i am not sure
- April 10, 2006 at 3:34 pm #45585
  canalon
  Participant
  More than that! I regularly amplify over 2 kb. with good conditions and enzymes, you can go way over that. Probably up to 10 kb.
- April 10, 2006 at 4:36 pm #45590
  Trev
  Participant
  it all depends from polymerase and PCR-method. My scientific leader amplified more than 16 kb.
- April 10, 2006 at 6:23 pm #45594
  LilKim
  Participant
  i’ve personally amplifed 8KB fragments. the problem is that regular taq has a high error rate (approx. 1 error/1000bp). Therefore, if fidelity is extremely important use hi-fi taq (hi fidelity taq)… which uses MgSO4 (instead of MgCL). Also.. the extension time has to be increased so that taq has enough time to extend….
- April 11, 2006 at 6:56 am #45645
  Helix
  Participant
  My sincere thanks to all who have responded to my post. Your answers and suggestions will be very much helpful to me.
Author
Posts

You must be logged in to reply to this topic.