Biology Forum Molecular Biology molecular biology

5 voices
5 replies
  • Author
    Posts
    • #4332
      Helix
      Participant

      Hi,
      does anyone know what is the maximum length of DNA that can be amplified by PCR? Please post the answer if some one knows.

    • #45568
      angel
      Participant

      may be its 1000 bp. i am not sure

    • #45585
      canalon
      Participant

      More than that! I regularly amplify over 2 kb. with good conditions and enzymes, you can go way over that. Probably up to 10 kb.

    • #45590
      Trev
      Participant

      it all depends from polymerase and PCR-method. My scientific leader amplified more than 16 kb.

    • #45594
      LilKim
      Participant

      i’ve personally amplifed 8KB fragments. the problem is that regular taq has a high error rate (approx. 1 error/1000bp). Therefore, if fidelity is extremely important use hi-fi taq (hi fidelity taq)… which uses MgSO4 (instead of MgCL). Also.. the extension time has to be increased so that taq has enough time to extend….

    • #45645
      Helix
      Participant

      My sincere thanks to all who have responded to my post. Your answers and suggestions will be very much helpful to me.

You must be logged in to reply to this topic.

Members